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摘要：目的 通過分析飛行員EDTA依賴性假性血小板減少癥,為準(zhǔn)確診斷血小板減少癥提供依據(jù). 方法 對(duì)1名用血細(xì)胞分析儀檢出血小板減少癥的飛行員,用手工法對(duì)血小板計(jì)數(shù)進(jìn)行復(fù)查,將EDTA與枸櫞酸、肝素抗凝血進(jìn)行對(duì)比,血涂片染色觀察血小板聚集情況. 結(jié)果 EDTA可誘發(fā)血小板聚集,導(dǎo)致假性血小板減少癥,可通過枸櫞酸、肝素抗凝血檢測(cè)進(jìn)行鑒別.采血后即刻用儀器檢驗(yàn)或手工檢驗(yàn),有助于避免假性診斷.經(jīng)手工核查排除假性血小板減少癥后,該飛行員被認(rèn)定飛行合格,飛行10h未發(fā)現(xiàn)出血現(xiàn)象和其他不適. 結(jié)論 對(duì)于飛行人員血小板減少癥,應(yīng)考慮EDTA誘導(dǎo)的假性血小板減少癥的可能.建議在采血即刻即進(jìn)行分析儀檢驗(yàn),或采用手工方法對(duì)血小板減少癥進(jìn)行復(fù)核,以此來(lái)避免誤診、誤治及錯(cuò)誤的飛行鑒定結(jié)論 . Abstract： Objective To provide evidence for accurately diagnosing thrombocytopenia in pilot by analyzing ethylene diamine tetreacetic acid (EDTA) dependent pseudothrombocytopenia.Methods A pilot was diagnosed as thrombocytopenia by EDTA count with hematology analyzer and his platelet was rechecked by manual test.The smears of citrate-and heparin-treated blood were taken for comparing the platelet aggregation. Results EDTA could induce platelet aggregation and cause pseudothrombocytopenia and that could be distinguished by the manual test of citrate-or heparin-treated blood.Testing of EDTA-treated blood by instrument shortly after sampling or manual test would be helpful to avoid such phenomenon. The pilot was qualified after re-examination by manual test of platelet.He was not found any hemorrhage and other discomfort in 10-hour flight. Conclusions EDTA dependent pseudo-thrombocytopenia may be resulted from instrument examination.Platelet counting immediately after blood sampling or rechecking by manual test for the flying personnel.who was diagnosed as thrombocytopenia by EDTA-treated blood counting,is suggested t

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